Biologist_PhD_15 years of experience

Cell Culture
• Culture and storage of mammalian (cancer) adherent and suspension cell lines
• Culture and storage of insect cell lines
• Isolation, culture and storage of human & mouse primary BM MSC
• Isolation and culture of mouse primary ECN, mouse cortical astrocytes and adult mouse SVZ- NPC
• Isolation, culture and storage of mouse primary tail fibroblasts
• Transwell coculture models
• Scratch wound assay (in vitro cell migration assay)
• Assessment of neuron death and survival (LDH, WST-1, MTT assay)
Molecular Biology and Biochemistry
• DNA, RNA and protein isolation from cells, tissues and human biospecimens
• Reverse Transcription PCR & Real-Time PCR
• Western blot
• Southern blot
• cDNA microarrays
• Slot blot
• Enzyme-linked Immunosorbent Assay (ELISA)
• Plasmid DNA purification
• Restriction enzyme digestion of DNA
Animal Models
• Characterization of transgenic and knockout mouse models
• Mouse husbandry and genotyping
• Mouse behavior monitoring
• Mouse tissue dissection and banking
• Histology (cryostat)
• Immunohisto- and Immunocytochemistry
• Induction and clinical scoring of CNS disease mouse models (multiple sclerosis-EAE and status epilepticus-kainic acid induced)
Microbiology
• Detection and isolation of viral strains (enteroviruses, measles virus, rubella virus & cytomegalovirus) from human biospecimens by cell culture
• Detection and isolation of enteroviruses from sewage samples by cell culture
• Detection of pathogen-specific antibodies in human serum specimens by immunofluorescence, microneutralization assay (CCID50), Western blot and ELISA
• Culture and storage of laboratory Helicobacter pylori strains
• Culture, antimicrobial susceptibility testing and storage of clinical Helicobacter pylori strains
Bright field and fluorescence microscopy
IT
• Statistical software- Sigma Plot, SigmaStat, GraphPad Prism
• PC applications- MS office, Adobe Photoshop etc.

ADDITIONAL SKILLS

• Study design, data statistical analysis, results interpreting
• Experience in writing and submitting papers for publication, preparing grant proposals, giving talks and poster presentations
• Familiarity with record-keeping
• Experience in working in Biological Safety Levels -2 and -3
• Training and supervision of undergraduate, MSc and PhD students

LANGUAGE SKILLS

• Greek (native speaker)
• English (fluent)
• German (good command)
• Italian (basic communication skills)