Qualifications
Research Work title-
Reserch tittle “Cloning, purification and characterization of two putative lipases, Rv1923 (LipD) and Rv2485c (LipQ) from Mycobacterium tuberculosis H37Rv and detection of its probable virulence” under supervision of Prof. Jagdeep Kaur, Head, Dept. of Biotechnology, Panjab University, Chandigarh
M.Sc. (Biotechnology):
(2002-2004), Second division, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India and secured 56%.
Bachelor in Life Sciences:
(2001), First division, Kurukshetra University, Kurukshetra, Haryana and secured 65%
10+2 Medical:
(1996) Second division, Haryana Board of School Education, Haryana and secured 56.0%.
Matriculation:
(1994)First division, Haryana Board of School Education, Haryana and secured 69% and
Technical Expertise
Well versed with all techniques used routinely in Immunology, Microbiology, Cell biology, Biochemistry and Molecular Biology.
Biochemistry and Molecular Biology: RNA, DNA and plasmid extraction, PCR amplification, Real-Time quantitative PCR, Semi-quantitative RT-PCR, SDS-PAGE, Western blotting, Molecular Cloning, Purification of recombinant protein, Affinity & Ion exchange chromatography, CD-spectra, Enzyme kinetics etc.
Microbiology: Various methods of antibiotic sensitivity testing, Minimum Inhibitory Concentration (MIC)
Virology: RNA isolation from Rotavirus
Cell Biology: Cell based assays (NO production estimation and phagocytosis), MTT assay, Cell cytotoxicity and proliferation assay, Maintaining cell lines of different origin.
Immunology: Polyclonal antibodies production, ELISA, Northern and Western Blot
Highlights Of Research Assignment
Tuberculosis (TB) is a major public health problem in India. India accounts for one-fifth of the global TB incident cases. Numerous genes have been implicated in the outcome of TB infection, leading to the characteristic variability of disease progression seen following TB infection. Extensively drug-resistant TB (XDR-TB) is resistant to first- and second-line drugs. Multidrug-resistant TB (MDR TB) is TB that is resistant to at least 2 of the best anti-TB drugs-isoniazid and rifampin. To prevent the continued emergence of drug-resistant strains of TB, treatment for TB must be improved.
M. tuberculosis is a bacterial pathogen that can persist for decades in an infected patient in dormant state without causing a disease. Actually, before entering in this state, bacteria accumulate lipids that are originating from the host cell membrane degradation and re-synthesis of complex lipid molecules. The presence of lipid inclusions confers the presence of lipolytic enzymes (lipases and/or phospholipases) capable of degrading these complexes.
During the reactivation phase of bacteria, these stored lipids are hydrolysed and the infection process occurs. Such lipid degradation pathway might contribute to the viability of mycobacteria during infection. Overall, lipases might not be the direct targets involved in pathogenesis but may contribute for the survival of the mycobacteria. Inhibition of these lipolytic enzymes offers opportunities for therapeutic interventions and immunemodulatory strategies.
Therefore, we decided to investigate the role of putative lipases by analyzing their effect on the production of NO in macrophages. The work was on Gene cloning, recombinant protein purification of Mycobacterial lipases (LipD and LipQ), biochemical and biophysical characterization of the enzymes and detection of their virulent effect by induction of Macrophages and analyzing the production of nitric oxide and expression of iNOS (induced nitric oxide synthase) gene. Also, I checked induction of these lipase genes under various stress conditions by Real-Time qRT-PCR.
Highlights Of Predoctoral Research Assignment
During M.Sc. dissertation I have worked on “M-Protein profiling of streptococci, RNA typing of Rotaviruses, Western Blotting of M-Protein & Diagnosis of Rotaviruses in feacal samples of equines by RNA Extraction method” during 4th semester of Master degree at National Research Center on Equines, Hisar. In this study we screened five different feacal samples for Rotaviruses by RNA extraction and northern blotting and for streptococci by extraction of M-protein and western blotting.
Besides this, I have worked in project on “Micro propagation of sugarcane” during 2th semester of Master degree at Harsac, Ch. Charan Singh Haryana Agriculture University, Hisar.
Computer Proficiency
I have working experience of different operating systems, windows. I am conversant with reports generation and power point presentations, MS-word, excel and basic bioinformatics techniques and web browsing.
Bioinformatics Tools: 3-D structure determination of proteins, Primer designing, BLAST; Nucleotide Sequence Analysis (BioEdit,); Multiple Sequence Alignment (CLUSTAL-W, BioEdit, MEGA; Phylogenetic analysis (PHYLIP, MEGA, DNAstar).
Career Summary
Seven Year Marketing Exp.
Verma Logistic ltd. Jammu
