Profile
Experienced, tenacious, and results-oriented hands-on background in (bio)sensors research and development and biotechnology of biologically active substances. Adept at working in an environment requiring problem resolution, with proven effectiveness to multitask in a competitive, high-impact, and fast-paced environment while juggling multiple priorities simultaneously with good judgment. Utilize strategic planning and decision-making skills in facilitating research to ensure efficiency. Capable of building rapport with all levels of professionals underscored by the ability to simplify and communicate complex scenarios. Articulate communicator; multilingual in English, Russian, and Ukrainian, with working knowledge of French, Czech, and German. Proficient with Microsoft Office Suite, Internet, as well as specialized databases and graphing software, Origin®.
Areas Of Expertise
Conductometric Sensors and Biosensors
Enzymes/Immobilized Enzymes
L-Arginine/Urea/Ammonium
Glucose/Lactose/Maltose/Saccharose
Heavy Metals
Real Sample Analysis
Analytical Chemistry
Biotechnology of Biologically Active Substances
Education
2009–2012
DOCTOR OF PHILOSOPHY IN ANALYTICAL CHEMISTRY AND BIOTECHNOLOGY
University Lyon 1, Graduate School, Lyon, France
2009–2012
DOCTOR OF PHILOSOPHY IN BIOTECHNOLOGY
National Academy of Sciences of Ukraine – Institute of Molecular Biology and Genetics, Graduate School, Kyiv, Ukraine
Thesis: Development and optimization of the novel conductometric (bio) sensors based on natural zeolite for ammonium, urea and L-arginine determination
2008–2009
MASTER OF SCIENCE IN BIOTECHNOLOGY WITH SPECIALIZATION IN BIOTECHNOLOGY OF BIOLOGICALLY ACTIVE SUBSTANCES
National University of Food Technologies, Kyiv, Ukraine
2004–2008
BACHELOR OF SCIENCE IN BIOTECHNOLOGY WITH SPECIALIZATION IN BIOTECHNOLOGY
National University of Food Technologies, Kyiv, Ukraine
Research Experience
National Academy of Sciences of Ukraine
Institute of Molecular Biology and Genetics, Kyiv, Ukraine
2015–Present
RESEARCH ASSOCIATE
Provide expert oversight to the research process on the development of the novel electrochemical sensors and biosensors for L-arginine determination
Develop protocols for immobilization of thiacalixarenes and calixarene phosphines on the gold supports as well as writing the laboratory instructions
Search optimum parameters of the working solutions for analytical performance of the developed sensors and biosensors
Preparation comprehensive research papers and reports, poster, and oral presentations
Take charge of preparing the patent applications
2012–2015
JUNIOR RESEARCHER
Perform experimentations on the development of the novel conductometric sensors and biosensors based on micro- and nanomaterials (zeolites and calixarenes) for L-arginine and ammonium determination
Develop biosensors and work protocols for application of the laboratory prototypes of the bioanalytical devices in the real sample analysis—medications, blood serum, and fruit juices
Evaluate and optimize analytical characteristics of the (bio)sensors for L-arginine and ammonium detection
Conduct thorough evaluation of the dependences of the analytical signals of the corresponding sensors and biosensors on the parameters of the working solutions
Draft comprehensive research papers and reports, poster, and oral presentations
Take charge of preparing the patent applications
2009
CHIEF ENGINEER
Administered the experiments on the development of the novel conductometric enzyme biosensors for determination of lactose and maltose; elaboration of the justified application procedures of the lactose biosensor in the milk quality control; and studies of the rate of the malt syrups aging using the maltose biosensor
Employed the methodology of the enzyme immobilization on the gold supports using the enzyme cross-linking with glutaraldehyde
Prepared sets of the model solutions and buffers for the biosensors’ calibration
Identified and assessed the effects of the working buffer parameters, including composition, pH value, buffer capacity, and ionic strength on the biosensors’ sensitivity, linear and dynamic ranges
Managed the immobilization of the bioselective elements of the biosensors
Prepared detailed research papers and reports as well as patent application
Training In Industry
2006 PLANT “STIROLBIOTECH”, the former “Trypil Biochemical Factory” , Obukhiv, Ukraine
2007 PLANT “TECHNOLOG”, a part of a group of pharmaceutical factories “Lekhim” , Uman, Ukraine
Professional Development
2014 IX SUMMER SCHOOL “MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY” (116 hours)
Odessa I.I. Mechnikov National University, Odessa, Ukraine
2011 LANGUAGE COURSES: “FRANÇAIS LANGUE ENTRANGERE DOCTORANTS” (36 hours)
University Lyon 1, Lyon, France
2010 LANGUAGE COURSES: “FRANÇAIS LANGUE ENTRANGERE SCIENCES” (30 hours)
University Lyon 1, Lyon, France
Affiliations
Union of Workers, National Academy of Sciences of Ukraine
Awards & Honors
Scholarship for Young Scientists: 2014-2015
National Academy of Sciences of Ukraine
Honors Diploma for the Best Poster Presentation: 2012
5th International Scientific and Technical Conference “Sensors Electronics and Microsystems Technology”
Honors Diploma for the Best Oral Presentation: 2010
4th International Scientific and Technical Conference “Sensors Electronics and Microsystems Technology”
Publications
Pyeshkova, V.M., Saiapina, O.Y., Soldatkin, O.O., Kukla, O.L., Dzyadevych, S.V. (2008). Enzyme conductometric biosensor for lactose content determination. Biotechnologia Acta, 1(4), 76–84.
Pyeshkova, V.M., Saiapina, O.Y., Soldatkin, O.O., Dzyadevych, S.V. (2009). Enzyme conductometric biosensor for maltose determination. Biopolymers and Cell, 25(4), 272–278.
Pyeshkova, V.M., Saiapina, O.Y., Soldatkin, O.O., Dzyadevych, S.V. (2010). Traditional and biosensor methods of mono- and disaccharides determination. Biotechnologia Acta, 3(3), 9–22.
Saiapina, O.Y., Dzyadevych, S.V., Jaffrezic-Renault, N., Soldatkin, O.P. (2012). Development and optimization of a novel conductometric bi-enzyme biosensor for L-arginine determination. Talanta, 92, 58–64.
Saiapina, O.Y., Dzyadevych, S.V., Walcarius, A., Jaffrezic, N. (2012). A novel highly sensitive zeolite-based conductometric microsensor for ammonium determination. Analytical Letters, 45(11), 1467–1484.
Saiapina, O.Y., Dzyadevych, S.V., Jaffrezic-Renault, N. (2012). Potentiality of application of the conductometric L-arginine biosensors for the real sample analysis. Biopolymers and Cell, 28(6), 441–448.
Saiapina, O.Y., Matsyshyn, M., Pyeshkova, V.M., Dzyadevych, S.V., Soldatkin, O.P., Melnik, V.G.,… Jaffrezic-Renault, N. (2012). Application of ammonium-selective zeolite for enhancement of conductometric bi-enzyme biosensor for L-arginine detection. Sensor Electronics and Microsystem Technologies, 3(9), 60–77.
Saiapina, O.Y., Pyeshkova, V.M., Soldatkin, O.O., Melnik, V.G., Akata Kurç, B., Walcarius, A.,…Jaffrezic-Renault, N. (2011). Conductometric enzyme biosensors based on natural zeolite clinoptilolite for urea determination. Materials Science and Engineering C, 31(7), 1490–1497.
Sheliakina, M., Arkhypova, V., Soldatkin, O., Saiapina, O., Akata, B., Dzyadevych, S. (2014). Urease-based ISFET biosensor for arginine determination. Talanta, 121, 18–23.
Soldatkin, A.P., Dzyadevych, S.V., Korpan, Y.I., Sergeyeva, T.A., Arkhypova, V.N., Biloivan, O.O.,… El’skaya, A.V. (2013). Biosensors. A quarter of a century of R&D experience. Biopolymers and Cell, 29(3), 188–206.
Soldatkin, O.O., Peshkova, V.M., Saiapina, O.Y., Kucherenko, I.S., Dudchenko, O.Y., Melnyk, V.G.,… Dzyadevych, S.V. (2013). Development of conductometric biosensor array for simultaneous determination of maltose, lactose, sucrose and glucose. Talanta, 115, 200–207.
Tekaya, N., Saiapina, O., Ben Ouada, H., Lagarde, F., Ben Ouada, F., Jaffrezic-Renault, N. (2013). Ultra-sensitive conductometric detection of heavy metals based on inhibition of alkaline phosphatase activity in Arthrospira platensis. Bioelectrochemistry, 90, 24–29.
Tekaya, N., Saiapina, O., Ben Ouada, H., Lagarde, F., Ben Ouada, H., Jaffrezic-Renault, N. (2013). Ultra-sensitive conductometric detection of pesticides based on inhibition of esterase activity in Arthrospira platensis. Environmental Pollution, 178, 82–188.
Tekaya, N., Saiapina, O., Ben Ouada, H., Lagarde, F., Ben Ouada, H., Jaffrezic-Renault, N. (2014). Bi-enzymatic conductometric biosensor for detection of heavy metal ions and pesticides in water samples based on enzymatic inhibition in Arthrospira platensis. Journal of Environmental Protection, 5, 441–453.
Patents
Pyeshkova, V.M., Saiapina, O.Y., Soldatkin, O.O., Dzyadevych, S.V. (2009). “Conductometric biosensor system for the determination of maltose concentration in a solution.” Ukraine Patent No. 43335.
Saiapina, O.Y., Dzyadevych, S.V., Soldatkin, O.P., Stasyuk, N.Y., Hayda, H.Z., Honchar, M.V. (2011). Conductometric biosensor system for the determination of L-arginine concentration on solution. Ukraine Patent No. 64025.
Oral Presentations
Gubchyk, O.M., Karpov, O.V., Saiapina, O.Y., Pyeshkova, V.M., Dzyadevych, S.V. (2010). Monitoring of saccharose and glucose concentration changes in molasses during its storage by means of enzyme conductometric biosensors. 76th Scientific Conference of Young Scientists, Doctorate Students and Students, “Scientific Achievement of the Youth for the Solution of Mankind Food Problems in the XXI Century”. Kyiv, Ukraine.
Pyeshkova, V.M., Saiapina, O.Y., Dzyadevych, S.V. (2008). Conductometric enzyme biosensor for lactose determination. 3rd International Scientific and Technical Conference, “Sensors Electronics and Microsystems Technology”. Odessa, Ukraine.
Peshkova,V.M., Saiapina, O.Y., Soldatkin, O.О., El’skaya, A.V., Soldatkin, A.P., Dzyadevych, S.V. (2008). Development of enzyme conductometric biosensors for determination of glucose, sucrose and lactose. Bridges in Life Sciences Second Annual Scientific Review Meeting. Zagreb, Croatia.
Peshkova, V.M., Saiapina, O.Y., Soldatkin, O.O., Melnik, V.G. (2009). Development of enzyme multibiosensor for carbohydrates determination. 4th Bridges in Life Sciences US-CEE Regional Networking Meeting. Debrecen, Hungary.
Pyeshkova, V.M., Saiapina, O.Y., Soldatkin, O.O., Dzyadevych, S.V (2009). Optimization of the conductometric enzyme multibiosensor’s system for simultaneous determination of sucrose, maltose, lactose, and glucose. 2nd International Conference, “Physical Methods in Ecology, Biology and Medicine”. Lviv-Vorokhta, Ukraine.
Pyeshkova, V.N., Saiapina, O.Y., Soldatkin, O.O., Dzyadevych, S.V. (2009). Development of biosensor for maltose determination. 5th International Conference “Functional Materials”. Partenit-Crimea, Ukraine.
Pyeshkova, V.M., Saiapina, O.Y., Dudchenko, O.Y., Melnyk, V.G., Semenycheva, L.N., Dzyadevych, S.V. (2012). Development of miniaturized enzyme biosensors with improved characteristics for simultaneous carbohydrates determination. 3rd International Meeting, “Clusters and Nanostructured Materials”. Uzhhorod-Vodohraj, Ukraine.
Saiapina, O.Y., Pyeshkova, V.M., Dzyadevych, S.V., Karpov, O.V. (2008). Investigation of the operation of an enzyme conductometric biosensor for lactose determination. 74th Scientific Conference of Young Scientists, Doctorate Students and Students, “Scientific Achievement of the Youth for the Solution of Mankind Food Problems in the XXI Century”. Kyiv, Ukraine.
Saiapina, O.Y., Pyeshkova, V.M., Dzyadevych, S.V., Karpov, O.V. (2009). Conductometric biosensor for maltose determination. 75th Scientific Conference of Young Scientists, Doctorate Students and Students, “Scientific Achievement of the Youth for the Solution of Mankind Food Problems in the XXI Century”. Kyiv, Ukraine.
Saiapina, O.Y., Pyeshkova, V.M., Dzyadevych, S.V. (2010). Zeolite nanoparticles for improvement of analytical characteristics of conductometric enzyme biosensor for urea determination. 4th Conference of Young Scientists, Illya Mechnikov 165th Anniversary. Kyiv, Ukraine.
Saiapina, O.Y., Pyeshkova, V.M., Jaffrezic-Renault, N., Dzyadevych, S.V. (2010). Investigation of conductometric biosensors for urea determination based on ammonium-sensitive natural zeolite. 4th International Scientific and Technical Conference, “Sensors Electronics and Microsystems Technology”. Odessa, Ukraine.
Saiapina, O.Y., Dzyadevych, S.V., Jaffrezic-Renault, N., Walcarius, A. (2010). Development of novel conductometric biosensors based on immobilized urease and clinoptilolite. Materiaux et Applications aux Dispositifs et Capteurs, 7èmes Journées Maghreb-Europe. Tabarka, Tunisia.
Saiapina, O.Y., Jaffrezic-Renault, N., Dzyadevych, S.V. (2011). Novel conductometric bienzyme biosensor for L-arginine determination based on arginase and urease. 5th Conference of IMBG Young Scientists, O.O. Bogomolets 130th Anniversary. Kyiv, Ukraine.
Saiapina, O.Y., Jaffrezic-Renault, N., Dzyadevych, S.V. (2011). Development and optimization of bienzyme conductometric biosensor for L-arginine determination. Journée Rhône-Alpes des Biomolécules. Lyon, France.
Saiapina, O.Y., Pyeshkova, V.M., Soldatkin, O.O., Melnik, V.G., Walcarius, A., Dzyadevych, S.V., Jaffrezic-Renault, N. (2011). Zeolite-based conductometric urea biosensors with improved sensitivity and stability. Journées d’Electrochimie. Grenoble, France.
Saiapina, O.Y., Dzyadevych, S.V., Walcarius, A., Jaffrezic-Renault, N. (2012). A novel conductometric microsensor based on natural zeolite for selective ammonium determination. 6th Young Scientists’ Conference of Institute of Molecular Biology and Genetics, NAS of Ukraine in Honor of the 155-year Anniversary of Sergei G. Navashin. Kyiv, Ukraine.
Saiapina, O.Y., Dzyadevych, S.V., Walcarius, A., Jaffrezic-Renault, N. (2012). Development of novel highly sensitive conductometric biosensors with natural zeolite as modifier for L-arginine determination. 5th International Scientific and Technical Conference, “Sensors Electronics and Microsystems Technology”. Odessa, Ukraine.
Saiapina, O.Y., Pyeshkova, V.N., Walcarius, A., Jaffrezic-Renault, N., Dzyadevych, S.V., Soldatkin, A.P. (2013). Clinoptilolite-containing conductometric biosensors for arginine determination. International Workshop, “Recent Advantages in Micro/Nano Sensors for Mono- and Multi-Target Assays”. Kyiv, Ukraine.
Saiapina, O., Shelyakina, M. Soldatkin, O., Arkhypova, V., Akata Kurç, Jaffrezic-Renault, B.N., Soldatkin, A., Dzyadevych, S.P. (2013). Application of clinoptilolite for enhancement of analytical characteristics of urease biosensors. 17th International Zeolite Conference. Moscow, Russia.
Saiapina, O.Y., Walcarius, A., Jaffrezic-Renault, N., Dzyadevych, S.V. (2014). Investigation of the ammonium ions adsorption on clinoptilolite for the material application as a selective element in the conductometric sensors. Conference of Young Scientists, “Current Issues in Biochemistry and Biotechnology – 2014”. Kyiv, Ukraine.
Saiapina, O.Y., Dzyadevych, S.V., Jaffrezic-Renault, N. (2014). Development and optimization of a clinoptilolite-based conductometric microsensor for selective determination of ammonium. 3rd National Scientific Conference, “Biotechnology: Achievements and Hopes”. Kyiv, Ukraine.
Saiapina, O.Y., Dzyadevych, S.V., Jaffrezic-Renault, N., Errachid, A., Walcarius, A. (2014). Clinoptilolite application in the conductometric biosensors. 8th Conference of Young Scientists of the Institute of Molecular Biology and Genetics of NASU. Kyiv, Ukraine.
Saiapina, O. (2015). А series of the works “Novel bioanalytical systems for medicine”. Interview for the National Radio Company of Ukraine, Promin Channel, Program “Azbuka Realnosti”. Kyiv, Ukraine.
Tekaya, N., Saiapina, O., Hatem, B., Lagarde, F., Hafedh, B., Jaffrezic-Renault, N. (2012). Etude d’un biocapteur basé sur la mesure d’activité enzymatique de cellules de la Spiruline pour la détection des métaux lourds. Materiaux et Applications aux Dispositifs et Capteurs, 8èmes Journées Maghreb-Europe. Sousse, Tunis.
