Highly Qualified Food Safety and Quality Assurance

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Serial No: 9961

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Current location:  Haryana, India - View on map

Nationality: Indian

Spoken languages: english, Hindi, punjabi

EDUCATION PROFILE

• Currently working as Research Associate in National Agriculture Innovative Project (NAIP) entitled “Development of Biosensor and Micro techniques for detection of Pesticide Residues, Aflatoxins, Heavy Metals and Bacterial Contaminants in milk”
• My doctoral work was “Development of enzyme substrate assay for monitoring E. coli in milk & Milk products”. It was focused mainly on development of analytical Micro-technique for rapid detection of food borne pathogen i.e. E. coli, E. coli O157:H7 and study its prevalence in Indian condition by screening raw, pasteurized, dried, cheese, ice-cream and some indigenous milk products like paneer, dahi and kulfi samples via developed and ISO certified conventional methods for isolation and enumeration of E. coli from dairy products.

Patents

• Balhara M, Kumar N, Thakur G, Vishweswaraiah H.R., Kumar V, Lawaniya R, Khan A and Kouser S. An enzyme-substrate assay for detection of Listeria monocytogenes in milk. Indian Patent no. 1357/DEL/2013
• Lawaniya R, Kumar N, Arora B, Khan A, Balhara M, and Vishweswaraiah H.R. Development of enzyme substrate assay for monitoring Escherichia coli in milk and milk products. Indian Patent filling under process at NDRI, Karnal.

Technology transfer:

• Technology on detection of L. monocytogenes was commercialized to M/s Neugen Diagnostic Pvt. Ltd., Hyderabad as Non-exclusive license during AGRI –TECH MEET held at NASC complex, New Delhi, India  from 18-19^th July 2013 

Papers

• Ramakant Lawaniya, Bhawna Arora, Mandeep Balhara, Naresh Kumar and Hirikyathanahalli Vishwesraiah Raghu. Screening of enrichment media for selective growth of E. coli. Journal of food safety (In Communication)

• Ramakant Lawaniya, Naresh Kumar, Alia Khan and Hirikyathanahalli Vishwesraiah Raghu Screening of Bacillus Spores for different Germinant to explore their role as biosensor. Journal of applied microbiology (In Communication)

• Umesh Kr. Shandilya,V. Panwar, S. Singh, R. Lawaniya and C.S. Pundir Kinetic properties of immobilized protease isolated from glycine max var.ogden. Journal of agriculture and animal sciences.

• Pradeep Kumar Sharma*, Pradeep Kumar Sharma, Sushil Kumar Shahi, Mahesh Kumar, Ramakant Lawaniya, Mandeep Balhara and Ranjeeta Sharma. Antimicrobial Potential of Ganoderma Fruiting Bodies and Cultured Mycelium. International Journal of Pharmacy and Pharmaceutical Sciences. (In Communication)

• Mandeep Balhara, Naresh Kumar, Hirikyathanahalli Vishwesraiah Raghu, Ramakant Lawaniya and Shabnam Kouser. Screening of enrichment media for selective growth of Listeria monocytogenes. Journal of food safety (In Press)

Review Article:

• Kumar N*, Thakur G, Raghu HV, Singh N, Sharma PK, Singh VK, Khan A, Balhara M, Avinash, Lawaniya R, Kouser S, Tehri N, Gopaul Rajesh and Shivani Arora (2013), Bacterial Spore Based Biosensor for Detection of Contaminants in Milk. J Food Process Technology, 4:11.

Oral presentation

• Lawaniya, R., Kumar, N., Arora, B., Khan, A., Balhara, M., Sharma P.K.,H.V. Raghu and Singh, V.K. Development of enzyme substrate assay for monitoring E. coli/ E. coli O157:H7 in milk. V international conference on Environmental, Industrial and applied microbiology. BioMicroWorld 2013 Madrid (Spain), 2-4 October 2013.

Poster presentation

• Khan, A., Kumar, N., Raghu H.V, Arora, S., Balhara, M., Lawaniya, R., Sharma P.K. and Singh, V.K. Enzyme-spore based chromogenic assay for monitoring antibiotic residues in milk. V international conference on Environmental, Industrial and applied microbiology. BioMicroWorld 2013 Madrid (Spain),2-4 October 2013
• Khan, A., Kumar, N., Lawaniya, R. and Raghu H.V. (2012). An innovative approach for detection of coliforms in milk based on spore germination. In XL Dairy industry conference held at New Delhi during 2-5 February, 2012.
• Lawaniya, R., Kumar, N. and Khan, A. (2012). Screening of Bacillus Spores for Different Germinant to Explore Their Role as Biosensor. In XL Dairy industry conference held at New Delhi during 2-5 February, 2012.
• Kumar, N., Khan, A. and Lawaniya, R. Application of bacterial spores as biosensors in detection of coliforms in milk. National seminar on value added dairy products at NDRI,Karnal,Haryana,India (2005)

Abstracts/ Popular articles in proceedings/ conferences/ affiliation

• Kouser S., Balhara M., Raghu H V., Kumar N., Thakur G., Lawaniya R., Khan A., Deshmukh  S. and Sharma P. K. (2013). Two stage enzyme assay for detection of Listeria monocytogenes in milk products. International Symposium on Frontier discoveries and innovations in microbiology and its interdisciplinary relevance (FDMIR-2013) during 17-20th Nov 2013 at MDU, Rohtak, India. Pp. 313.
• Participated in V international conference on Environmental, Industrial and applied microbiology. BioMicroWorld 2013 Madrid (Spain), 2-4 October 2013.
• Balhara, M., Lawaniya, R. and Kumar, N. (2011) Development of Enzyme Substrate Based Optical Assay for Detection of Listeria monocytogenes in Milk and Milk products. (In- National seminar on Recent Advances in the development of fermented foods. Organized by center of food sciences and technology, Institute of agricultural sciences. Banaras Hindu University, Varanasi, India) (Accepted for poster presentation)
• Participated in international conference on Probiotics for Human Health: New innovations and emerging trends, at India Habitat Centre, New Delhi during August 27-28,2012
• Participated in international conference on Fundamental dairy foods held at National Dairy Research Institute, Karnal during November 16-19, 2011.
• Participated in National Symposium/Workshop on new trends of Biosensor Technology (NSNTBT-2009). Held at Department of Physics, Hindustan College of Science and Technology, Farah, Mathura, India during January 17-19, 2009.
• Membership of Indian Dairy Association (IDA)
• Life Time member of Probiotic Association Of India (PAI)
• Membership of American Dairy Science Association (ADSA) 

Manual Editing and Compilation

• Kumar, N., Raghu H.V., Puniya, A.K., Lawaniya, R., Balhara, M., Kumar,V., Arora, B and Dahiya, M. (Preparation, Editing and compilation of lecture compendium on Conventional and Rapid Microbiological Techniques for Quality and Safety Monitoring in Dairy Industry) held on 25^th -30^th July 2011. N.D.R.I, Karnal.
• Kumar, N., Raghu, H.V, Malik, R.K., Lawaniya, R., Balhara, M and Kumar, V. (Preparation editing and compilation of Biosafety Manual – A Holistic Approach to Biosafety in Dairy Industry) 1^st ed. March 2012. N.D.R.I, Karnal.

Manual chapters

• Kumar, N., Lawaniya, R., Balhara, M. and Malik, R.K. (2009). Milk as a potential carrier of spoilage and pathogenic bacteria and their safety concerns. (In Training programme on fundamental aspects of Dairy microbes and their safety concerns in dairy industry, held on 29 -30 September. N.D.R.I, Karnal.

• Raghu, H. V. Lawaniya, R. & Avinash (2011). Principle and procedure for direct and indirect tests for enumeration of E. coli/E. coli O157:H7, Salmonella/Shigella. (In Training Programme on Conventional and Rapid Microbiological Techniques for Quality and Safety Monitoring in Dairy Industry, held on 25-30 July). N.D.R.I, Karnal.

• Lawaniya, R., Avinash & Raghu H V.(2011). Procedure for preparation of non-selective, selective and differential media for enumeration of E. coli/E. coliO157:H7, Salmonella/Shigella. (In Training Programme on Conventional and Rapid Microbiological Techniques for Quality and Safety Monitoring in Dairy Industry, held on 25-30 July). N.D.R.I, Karnal.

• Lawaniya, R. and Kumar, N. (2009). Conventional and Rapid Methods for Detection of E. coli in Dairy Products
• Khan, A. and Lawaniya, R. (2012) Emergency Procedures (In Manual-A Holistic Approach to Biosafety in Dairy Industry) 1^st ed. March 2012. N.D.R.I, Karnal.
• Lawaniya, R. and Sharma, P.K. (2012) Biosafety Officer and Biosafety committee Procedures (In Manual-A Holistic Approach to Biosafety in Dairy Industry) 1^st ed. March 2012. N.D.R.I, Karnal.
• Balhara, M., Lawaniya, R., Khan,A.,Singh,N.A.,Sharma,P.K. and Yadav,A. (2012) Equipment Handling and Microbial Biosafety Techniques (In Manual-A Holistic Approach to Biosafety in Dairy Industry) 1^st ed. March 2012. N.D.R.I, Karnal.

TECHNIQUES ACQUAINTED WITH

Microbiological Techniques: Isolation and characterization of bacteria using conventional cultural, biochemical, and serological methods. Revival of cultures, their storage and maintenance

Sample analysis for E. coli, Coliforms, Listeria spp. using Petri Film Reader

Detection of pathogens (E. coli O157H7, Listeria monocytogenes) using VIDAS system (INTERNATIONAL VALIDATION by AOAC, AFNOR and NORDVAL), D-count (Biomerieux, France) and BIACORE 3000 (Flowcytometry Based)

Identification of bacteria (E. coli, E. coli O157:H7, Listeria monocytogenes, Enterococcus faecalis, Enterococcus faecium) using API kits. Using fluorogenic markers for specific detection of bacterial pathogen on Microbiological Plate Reader (Perkin Elmer Victor X-3) 

Instrumentation: Basic knowledge of the operation and handling of the EMCCD camera, Microbiological Plate Reader (Perkin Elmer Victor -3), VIDAS system (INTERNATIONAL VALIDATION by AOAC, AFNOR and NORDVAL), D-count (Biomerieux, France) and BIACORE 3000 (Surface Plasmon Resonance Based), Charm Analyzer 6602, Rosa Reader, 3M scientific Petri Film Reader,  Biosafety cabinet level-2 in BSL-2 Laboratory facility

Microscopy: Bright field, Phase contrast, Fluorescent 

Other: Qualitative and quantitative enzyme assays for Alpha-Galactosidase, Beta-Galactosidase, Beta-glucuronidase, Beta-Glucosidase, Tryptophanase, Alpha-mannosidase, and Phosphatidylinositol specific phospholipase- C (PI-PLC) and study their potential as markers enzymes in specific identification of food borne pathogens.